AffiBEADS® Anti-MBP Nanobody Affinity Agarose Background
MBP (Maltose Binding Protein) is a commonly used affinity tag, which can increase the expression level and solubility of the target protein. Anti-MBP affinity gel is made by covalently coupling high-quality MBP nanobody with agarose gel. Since the nanobody only contains the variable region of Antibody molecules, there will be no signal interference from the heavy and light chains of Antibody during immuno (co) precipitation. It has the characteristics of high binding capacity of protein, high specificity and stability, and can be used for immuno (co) precipitation of MBP tagged fusion protein.
Coupled Ligand
Anti-MBP nanobody.
Binding Capacity
1.2 mg protein /mL agarose.
Beads
Sepharose 4B agarose with average particle size of 50 μm.
Conjugate
Agarose.
Antibody Isotype
VHH.
Main Biomaterial Source
Alpaca.
Product Category
Affinity Gel/IP-CoIP.
Recommended slurry Volume Per Run
40 μL.
Storage Buffer
PBS with preservative and 50% glycerol, pH7.2.
Storage Conditions
(-20°C) for 12 months.